INVESTIGATOR: Wang, Jianping; Glynn, Neil; Gilbert, Robert
COLLABORATORS: Schnell, Raymond; Binder, Joseph
INSTITUTION: University of Florida
NON-TECHNICAL SUMMARY: The southern US with its long growing season and high light intensity is a potentially significant contributor to the national goal of biomass production. In the sub-tropical regions of the US, energy cane (Saccharum complex hybrids) holds great potential as a bioenergy feedstock. The goal of this project is to improve energy cane biomass production through genetic and genomic analysis of an evaluated and selected Saccharum germplasm collection to identify the genetic components contributing to biomass production. The project will produce a range of foundational genetic resources and genetic makers for energy cane breeders to efficiently develop energy cane cultivars with increased biomass production and reduced input requirement.
OBJECTIVES: 1. Identify a core collection of approximately 250 accessions from the World Collection of Sugarcane and Related Grasses held at USDA-ARS Miami, FL that captures the greatest extent of molecular and phenotypic diversity. 2. Discover desirable alleles contributing to biomass composition in Saccharum spp. through association analysis between allelic variability in candidate genes and lignocellulosic biomass components and cell wall composition. 3. Develop energy cane cultivars through marker assisted selection.
APPROACH: For objective 1) phenotypic and genotypic evaluation of all of the accessions in the world Saccharum germplasm collection. A set of accessions, representing most of the overall genetic variance of the collection will be selected to form a core collection. For objective 2), allele variants of candidate genes contributing to lignocellulosic biomass will be obtained from amplification and next generation sequencing. Biomass and bioenergy traits will be evaluated on the core collection using field and laboratory measurements. Association analysis between the phenotypic and genotypic variation will be performed to identify alleles associated to targeted biomass traits that can be used for genotype selection. For objective 3), the desirable alleles contributing to biomass will be converted to genetic markers to assist the selection of energy cane breeding lines in the energy cane breeding program. The selected lines will be further evaluated and characterized in the field.
Name: Wang, Jianping